BP701T. INSTRUMENTAL METHODS OF ANALYSIS (Theory)

UNIT –I           

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

UNIT –II    

IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

Atomic absorption spectroscopy– Principle, interferences, instrumentation and applications

Nepheloturbidometry– Principle, instrumentation and applications

UNIT –III        

Introduction to chromatography

Adsorption and partition column chromatography-Methodology, advantages, disadvantages and applications.

Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.

Paper chromatography-Introduction, methodology, development    techniques, advantages, disadvantages and applications

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications

UNIT –IV    

Gas   chromatography    –    Introduction,   theory,   instrumentation,    derivatization, temperature programming, advantages, disadvantages and applications

High performance liquid chromatography (HPLC)-Introduction, theory, instrumentation, advantages and applications.

UNIT –V      

Ion exchange chromatography- Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications

Gel chromatography- Introduction, theory, instrumentation and applications

Affinity chromatography- Introduction, theory, instrumentation and applications